|Synonyms||PDGF-2, GDGF, ODGF, SIS, SSV|
|Description||Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.
Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 4 × 105 units/mg.|
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV
WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR
KKPIFKKATV TLEDHLACKC ETIVTPRPVT
|Measured Molecular Weight||24.7 kDa, observed by non-reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
For more documents, please visit "Technical Support".