|Synonyms||Fibroblast growth factor 18, FGF-18, zFGF5, Fgf18, D130055P09Rik|
|Description||Fibroblast Growth Factor 18 (FGF-18) is a pleiotropic cytokine belonging to the heparin-binding FGF family, which has 23 different members. Structurally, FGF-18 is closely related to FGF-8 and FGF-17. Like other FGFs, FGF-18 can bind to different FGF receptors in vivo. FGF-18 is expressed in various tissues and has multiple functions: during long bone growth, FGF-18 is expressed in perichondrium and developing joints, and regulates bone formation by inhibiting chondrocyte proliferation and differentiation; FGF-18 knock-out mice survive embryonic development, but exhibit skeletal abnormalities and die in the early neonatal period. FGF-18 also induces ectopic cartilage formation in the lung, and alters the morphology of the pulmonary mesenchyma.
Recombinant rat Fibroblast Growth Factor 18 (rrFGF-18) produced in E. coli is a single non-glycosylated polypeptide chain containing of 172 amino acids. A fully biologically active molecule, rrFGF-18 has a molecular mass of 20.1 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5 µg/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2× 103 units/mg.|
EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ
VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY
LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS
GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK
|Measured Molecular Weight||20.1 kDa, observed by non-reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant rat Fibroblast Growth Factor 18 (rrFGF-18) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrFGF-18 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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