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IL-11, Human(CHO-expressed)

Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 has multiple effects on both hematopoietic and non-hematopoietic cells. Many of the biological effects described for IL-11 overlap with those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells.
$100.00
Z03108-5

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Product Introduction
Species Human
Protein Construction
IL-11 (Pro22-Leu199)
Accession # P20809-1
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 5.0 ng/ml, measured in a proliferation assay using TF-1 cells.
Expression System CHO
Apparent Molecular Weight ~23 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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IL-11, Human(CHO-expressed)

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IL-11, Human(CHO-expressed)

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IL-11, Human(CHO-expressed)

Biological Activity
IL-11, Human (Cat. No. Z03108) stimulates cell proliferation of T11 cells. The ED50 for this effect is less than 5ng/mL. »

IL-11, Human(CHO-expressed)

ED50 < 5.0 ng/ml, measured in a proliferation assay using TF-1 cells. »

IL-11, Human(CHO-expressed)

Lane 1: 2 μg of IL-11, Human(CHO-expressed), reducing (R)
Lane 2: 2 μg of IL-11, Human(CHO-expressed), non-reducing (NR)
> 95% as analyzed by SDS-PAGE »

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Background
Target Background Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 has multiple effects on both hematopoietic and non-hematopoietic cells. Many of the biological effects described for IL-11 overlap with those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells.
Synonyms IL11; AGIF; IL-11; interleukin 11
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.