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Catalog Products » Recombinant Proteins » Cytokines » Interleukin-3 (IL-3), Mouse

IL-3, Mouse

Synonyms MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Description Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family.
Recombinant mouse Interleukin-3 (rmIL-3) produced in E. coli is a single non-glycosylated polypeptide chain containing of 135 amino acids. A fully biologically active molecule, rmIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P01586
Species Mouse
Source E. coli
Specific Activity ED50 < 0.05 ng/mL, measured by a cell proliferation assay using M-NFS-60 cells, corresponding to a specific activity of > 2 × 107 units/mg.
Sequence
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK
SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA
NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ
PASGSVSPNR GTVEC

Measured Molecular Weight 15.2 kDa, observed by non-reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Interleukin-3 (rmIL-3) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-3 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • IL-3, Mouse
  • IL-3, Mouse

    ED₅₀< 0.05 ng/mL, measured by a cell proliferation assay using M-NFS-60 cells, corresponding to a specific activity of > 2 × 10⁷ units/mg.




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Cat. No. Z03111
Size
Price
50 ug $275.00
10 ug $85.00
1 mg $2100.00
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