|Synonyms||Granulocyte, Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFβ, pluripoietin|
|Description||Granulocyte Colony-Stimulating Factor (G-CSF) is a hematopoietic cytokine belonging to the four-helix bundle cytokine superfamily. G-CSF is produced by monocytes, macrophages, fibroblasts, and endothelial cells. Its expression is highly regulated and induced by a variety of agents, including Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Interferon γ (IFN-γ), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). G-CSF binds to the CSF-specific high affinity receptors expressed on neutrophilic granulocyte lineage. In vivo G-CSF regulates the production of neutrophilic granulocytes, a critical part of host defense systems, and helps the maturation of leukemic cell lines. G-CSF is widely employed clinically because of its fairly innocuous safety profile.
Recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) produced in E. coli is a single non-glycosylated polypeptide chain containing of 194 amino acids. A fully biologically active molecule, rrG-CSF has a molecular mass of 21.4 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.1 ng/mL, measured by a cell proliferation assay using NSF-60 cells, corresponding to a specific activity of > 1 × 107 units/mg.|
MIPLLTVSSL PPSLPLPRSF LLKSLEQVRK IQARNTELLE
QLCATYKLCH PEELVLFGHS LGIPKASLSS CSSQALQQTK
CLSQLHSGLF LYQGLLQALA GISSELAPTL DMLHLDVDNF
ATTIWQQMES LGVAPTVQPT QSTMPIFTSA FQRRAGGVLV
TSYLQSFLET AHHALHHLPR PAQKHFPESL FISI
|Measured Molecular Weight||21.4 kDa, observed by non-reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against 20mM Citric Acid|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrG-CSF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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