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Catalog Products » Recombinant Proteins » Cytokines » TRAIL/Apo2L, Human

TRAIL/Apo2L, Human

Synonyms TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand, TL2
Description TRAIL/Apo2L, also known as Tumor Necrosis Factor Super-Family 10 (TNFSF10), is a pleiotropic cytokine thatbelongs to the TNF superfamily. The full length TRAIL expressed in vivo is a Type II transmembrane protein, although the soluble form also exists and functions. TRAIL has four major receptors: two death receptors DR4 and DR5, two decoy receptors DcR1 and DcR2. TRAIL binds to the death receptors, recruits the FAS-associated death domain, activates caspases 8 and 10, and eventually leads to apoptosis. Because of its antitumor potential, TRAIL is actively studied as a therapeutic agent. On the other hand, abnormal expression of TRAIL in small arteries can induce the proliferation of smooth muscle cells, resulting in increasing vascular remodeling and pulmonary arterial hypertension.
Recombinant human TRAIL/Apo2L (rhTRAIL) produced in E. coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhTRAIL has a molecular mass of 19.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P50591
Species Human
Source E. coli
Biological Activity ED50 < 40 ng/mL, measured by the cell growth inhibitory assay using RPMI-8226 cells, corresponding to a specific activity of > 2.5 × 104 units/mg.
Sequence
MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN
SWESSRSGHS FLSNLHLRNG ELVIHEKGFY YIYSQTYFRF
QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS
KDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA
SFFGAFLVG

Measured Molecular Weight 19.6 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human TRAIL/Apo2L (rhTRAIL) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhTRAIL remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only

  • TRAIL/Apo2L, Human
  • TRAIL/Apo2L, Human

    ED₅₀< 40 ng/mL, measured by the cell growth inhibitory assay using RPMI-8226 cells, corresponding to a specific activity of > 2.5 × 10⁴units/mg.




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Cat. No. Z03124
Size
Price
50 ug $85.00
10 ug $39.00
1 mg $559.00