|Description||Fibroblast Growth Factor-12(FGF-12) is a heparin binding cytokine belonging to the FGF family. FGF-12 along with FGF-11, -13, and -14, form a sublineage within the FGF family: in contrast to the other members, they are all intracellular signaling proteins lacking signal peptides and containing a flanking domain beside the family conserved β-trefoil domain. FGF-12 is expressed in the cartilaginous skeleton and heart, suggesting a role in the development of connective tissue and heart. In vivo, FGF-12 binds to Islet Brain-2 and Voltage-Gated Sodium Channels (VGSC), and plays a critical role in the membrane targeting and function of VGSC. FGF-12 has been implicated in heart diseases such as cardiac arrhythmias.
Recombinant human Fibroblast Growth Factor-12 (rhFGF-12) produced in E. coli is a single non-glycosylated polypeptide chain containing 181 amino acids. A fully biologically active molecule, rhFGF-12 has a molecular mass of 20.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 20 ng/ml, measured by its binding ability in a functional ELISA with immobilized rhFGF R4/Fc Chimera, corresponding to a specific activity of > 5×104 units/mg.|
MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD
YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT
PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ
IMKGNRVKKT KPSSHFVPKP IEVCMYREPS LHEIGEKQGR
SRKSSGTPTM NGGKVVNQDS T
|Measured Molecular Weight||20.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-12 (rhFGF-12) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-12 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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