> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
|Endotoxin Level||< 0.2 EU/μg of protein by gel clotting method|
|Biological Activity||ED50 < 200.0 ng/ml, measured cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5.0 × 103 units/mg.|
|Expression System||E. coli|
|Apparent Molecular Weight||~22.8 kDa, on SDS-PAGE under reducing conditions.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.|
|Storage & Stability||Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
|Target Background||Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor β subunit, and a CNTF specific α receptor subunit. Upon binding to the CNTF, the β subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons.|
|Synonyms||Ciliary Neurotrophic Factor|
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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