|Synonyms||Ciliary Neurotrophic Factor|
|Description||Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor β subunit, and a CNTF specific α receptor subunit. Upon binding to the CNTF, the β subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons.
Recombinant human Ciliary Neurotrophic Factor (rhCNTF) produced in E. coli is a single non-glycosylated polypeptide chain containing 199 amino acids. A fully biologically active molecule, rhCNTF has a molecular mass of 22.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 200 ng/ml, measured cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5×103 units/mg.|
AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTESYVKH
QGLNKNINLD SADGMPVAST DQWSELTEAE RLQENLQAYR
TFHVLLARLL EDQQVHFTPT EGDFHQAIHT LLLQVAAFAY
QIEELMILLE YKIPRNEADG MPINVGDGGL FEKKLWGLKV
LQELSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM
|Measured Molecular Weight||22.8 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Ciliary Neurotrophic Factor (rhCNTF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhCNTF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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