|Synonyms||Insulin-like Growth Factor-II, Somatamedin A|
|Description||Insulin-like Growth Factor II (IGF-II) is a single chain 7 kDa polypeptide, and shares a high degree of homology with insulin. During circulation in vivo, IGF-II is complexed to high affinity binding proteins, IGF Binding Proteins (IGFBP), which act as circulating reservoirs, transport IGF-II, and prolong the half life of IGF-II. The receptors of IGF-II (IGFRs) are transmembrane tyrosine receptors, and are heterotetrameric consisting of two α-subunits and two β-subunits. IGFRs execute their role via intracellullar signaling molecules, such as IRS, shc, and PI3K. The functions of IGF-II include promoting cell survival, growth, proliferation, differentiation and motility. In particular, IGF-II promotes proliferation, inhibits death, and stimulates transformation in breast cancer cells.
Recombinant human Insulin-like Growth Factor II (rhIGF-II) produced in E. coli is a single non-glycosylated polypeptide chain containing 68 amino acids. A fully biologically active molecule, rhIGF-II has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 20 ng/mL, measured by a cell proliferation assay using FDCP-1 cells, corresponding to a specific activity of > 5×104 units/mg.|
MAYRPSETLC GGELVDTLQF VCGDRGFYFS RPASRVSRRS
RGIVEECCFR SCDLALLETY CATPAKSE
|Measured Molecular Weight||7.6 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against 25mM Tris, pH8.0.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Insulin-like Growth Factor II (rhIGF-II) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIGF-II remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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