|Synonyms||Fibroblast Growth Factor-21, FGFL|
|Description||Fibroblast Growth Factor-21 (FGF-21) is a metabolic cytokine belonging to the heparin-binding FGF family. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. In vivo FGF-21 is expressed in liver, pancreas, adipose tissue, and skeletal muscle, and it plays a central role in the energy metabolism. The expression of FGF-21 is stimulated by free fatty acids and insulin resistant states and is correlated with whole-body insulin resistance. FGF-21 activates glucose uptake in adipocytes and increases insulin sensitivity, implicating it as a novel target with potential anti-diabetic properties.
Recombinant human Fibroblast Growth Factor-21 (rhFGF-21) produced in E. coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rhFGF-21 has a molecular mass of 19.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5 µg/mL, measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 µg/mL mouse Klotho and 10 µg/mL heparin, corresponding to a specific activity of > 2× 103 units/mg.|
GHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG
TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD
GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP
GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD
VGSSDPLSMV GPSQGRSPSY AS
|Measured Molecular Weight||19.5 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-21 (rhFGF-21) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-21 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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