|Description||Fibroblast Growth Factor 8a (FGF-8a) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, and thus participate in different signaling cascade pathways. FGF-8 has very widespread expression during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression, but FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.
Recombinant human Fibroblast Growth Factor 8a (rhFGF-8a) produced inE. coli is a single non-glycosylated polypeptide chain containing 183 amino acids. A fully biologically active molecule, rhFGF-8a has a molecular mass of 21.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 500 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/ml of heparin, corresponding to a specific activity of > 2× 103 units/mg.|
MQHVREQSLV TDQLSRRLIR TYQLYSRTSG KHVQVLANKR
INAMAEDGDP FAKLIVETDT FGSRVRVRGA ETGLYICMNK
KGKLIAKSNG KGKDCVFTEI VLENNYTALQ NAKYEGWYMA
FTRKGRPRKG SKTRQHQREV HFMKRLPRGH HTTEQSLRFE
FLNYPPFTRS LRGSQRTWAP EPR
|Measured Molecular Weight||21.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor 8a (rhFGF-8a) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-8a remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
For more documents, please visit "Technical Support".