• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-8a (FGF-8a), Human

FGF-8a, Human

Synonyms AIGFa; HBGF-8a
Description Fibroblast Growth Factor 8a (FGF-8a) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, and thus participate in different signaling cascade pathways. FGF-8 has very widespread expression during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression, but FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.
Recombinant human Fibroblast Growth Factor 8a (rhFGF-8a) produced inE. coli is a single non-glycosylated polypeptide chain containing 183 amino acids. A fully biologically active molecule, rhFGF-8a has a molecular mass of 21.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P55075
Species Human
Source E. coli
Biological Activity ED50 < 500 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/ml of heparin, corresponding to a specific activity of > 2× 103 units/mg.
Sequence
MQHVREQSLV TDQLSRRLIR TYQLYSRTSG KHVQVLANKR
INAMAEDGDP FAKLIVETDT FGSRVRVRGA ETGLYICMNK
KGKLIAKSNG KGKDCVFTEI VLENNYTALQ NAKYEGWYMA
FTRKGRPRKG SKTRQHQREV HFMKRLPRGH HTTEQSLRFE
FLNYPPFTRS LRGSQRTWAP EPR

Measured Molecular Weight 21.3 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Fibroblast Growth Factor 8a (rhFGF-8a) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-8a remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. Z03172
Size
Price
10 ug $65.00
50 ug $159.00
1 mg $1500.00