|Synonyms||Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5)|
|Description||Neurotrophin-4 (NT-4) is a small secreted cytokine, and belongs to the Neurotrophin (NT) family, which also includes Brain Derived Neurotropic Factor (BDNF), Nerve Growth Factor (NGF), and NT-3. NT family members are all derived from similar sized protein precursors, composed of N-terminal propeptides and C-terminal mature domains, which are separated by posttranslational proteolytic cleavage. NT-4 (along with NT-3) is foundin the brains of mammals . In vivo, NT-4 binds to the common receptor, p75NTR, and a tyrosine kinase receptor, TrkB. The heterotrimeric complex activates the NFκB transcription factor. NT-4 is essential for the differentiation and wiring regulation of the central and peripheral nervous systems during development, and is related to important diseases including Alzheimer’s.
Recombinant mouse Neurotrophin-4 (rmNT-4) produced in E. coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chainsof 131 amino acids. A fully biologically active molecule, rmNT-4 has a molecular mass of 14.0kDaanalyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 <1µg/mL, measured by a cell proliferation assay usingC6cells, corresponding to a specific activity of>1× 103 units/mg.|
MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE
VLGEVPAAGG SPLRQYFFET RCKAESAGEG GPGVGGGGCR
GVDRRHWLSE CKAKQSYVRA LTADSQGRVG WRWIRIDTAC
|Measured Molecular Weight||14.0 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGEanalysis.|
|Formulation||Lyophilized after extensive dialysis against 50mM acetic acid.|
|Reconstitution||Reconstituted in 50mM acetic acid or ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Neurotrophin-4 (rmNT-4) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmNT-4 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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