• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Other Catalog Proteins » Influenza A H1N1 (A/California/04/2009 (H1N1)), His, Hemagglutinin

H1N1 (A/California/04/2009), Hemagglutinin

Synonyms HA
Description Influenza hemagglutinin (HA) is a glycoprotein found on the surface of the influenzavirus. It is responsible for binding the virus to cells with sialic acid on their membranes, such as cells in the upper respiratory tract or erythrocytes. It is also responsible for the fusion of the viral envelope with the endosome membrane after the pH has been reduced. The name "hemagglutinin" comes from the protein's ability to cause red blood cells (erythrocytes) to clump together in vitro. HA has two functions. First, it allows the recognition of target vertebrate cells, accomplished through binding to these cells' sialic acid-containing receptors. Second, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. H1N1 is a subtype of influenza virus A and the most common cause of influenza in humans.
Recombinant Influenza A H1N1 (A/California/04/2009(H1N1)) Hemagglutinin with his-tag produced in Sf9 Cell is a single, glycosylated polypeptide chain containing 520 amino acids. A fully biologically active molecule, HA-H1N1 has a molecular mass of ~66 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No ACP41105.1
Species Hemagglutinin
Source Sf9 insect cells
Biological Activity This recombinant protein has not been tested.
Sequence
MKAILVVLLY TFATANADTL CIGYHANNST DTVDTVLEKN
VTVTHSVNLL EDKHNGKLCK LRGVAPLHLG KCNIAGWILG
NPECESLSTA SSWSYIVETP SSDNGTCYPG DFIDYEELRE
QLSSVSSFER FEIFPKTSSW PNHDSNKGVT AACPHAGAKS
FYKNLIWLVK KGNSYPKLSK SYINDKGKEV LVLWGIHHPS
TSADQQSLYQ NADTYVFVGS SRYSKKFKPE IAIRPKVRDQ
EGRMNYYWTL VEPGDKITFE ATGNLVVPRY AFAMERNAGS
GIIISDTPVH DCNTTCQTPK GAINTSLPFQ NIHPITIGKC
PKYVKSTKLR LATGLRNIPS IQSRGLFGAI AGFIEGGWTG
MVDGWYGYHH QNEQGSGYAA DLKSTQNAID EITNKVNSVI
EKMNTQFTAV GKEFNHLEKR IENLNKKVDD GFLDIWTYNA
ELLVLLENER TLDYHDSNVK NLYEKVRSQL KNNAKEIGNG
CFEFYHKCDN TCMESVKNGT YDYPKYSEEA KLNREEIDGV
KLESTRIYQH HHHHHHH

Measured Molecular Weight ~66 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized in 20 mM PB buffer (pH 7.4), 300 mM NaCl, 5% mannitol, 5% trehalose.
Reconstitution Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower.
Endotoxin Level < 1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Influenza A H1N1 (A/California/04/2009(H1N1)) Hemagglutinin remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, HA-H1N1 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. Z03181
Size
Price
10 ug $39.00
100 ug $195.00