|Synonyms||Serpin A12, OL-64, Visceral adipose tissue-derived serine protease inhibitor|
|Description||Vaspin is a cytokine originally identified in visceral adipose tissue of Otsuka Long-Evans Tokushima fatty rats, and the name “Vaspin” is short for visceral adipose tissue-derived serine protease inhibitor. Besides the visceral adipose, Vaspin is also expressed in the skin, hypothalamus, pancreatic islets and stomach, and is shown to exert an anti-inflammatory role by inhibiting several proinflammatory adipokines such as leptin, resistin, and Tumor Necrosis Factor-α. Vaspin also stimulates adiponectin expression and improves insulin sensitivity in mice. Vaspin expression has been shown to decrease with worsening of diabetes and body weight loss. Accordingly, administration of recombinant human Vaspin improved glucose tolerance in diet regulated mice suggesting it as a potential target for obese-related diseases.
Recombinant human Vaspin (rhVaspin) produced in E. coli is a single non-glycosylated polypeptide chain containing 394 amino acids. rhVaspin has a molecular mass of 45.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Specific Activity||Bioassay data are not available.|
LKPSFSPRNY KALSEVQGWK QRMAAKELAR QNMDLGFKLL
KKLAFYNPGR NIFLSPLSIS TAFSMLCLGA QDSTLDEIKQ
GFNFRKMPEK DLHEGFHYII HELTQKTQDL KLSIGNTLFI
DQRLQPQRKF LEDAKNFYSA ETILTNFQNL EMAQKQINDF
ISQKTHGKIN NLIENIDPGT VMLLANYIFF RARWKHEFDP
NVTKEEDFFL EKNSSVKVPM MFRSGIYQVG YDDKLSCTIL
EIPYQKNITA IFILPDEGKL KHLEKGLQVD TFSRWKTLLS
RRVVDVSVPR LHMTGTFDLK KTLSYIGVSK IFEEHGDLTK
IAPHRSLKVG EAVHKAELKM DERGTEGAAG TGAQTLPMET
PLVVKIDKPY LLLIYSEKIP SVLFLGKIVN PIGK
|Measured Molecular Weight||45.1 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Vaspin (rhVaspin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhVaspin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
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