|Description||Fibroblast Growth Factor 8f (FGF-8f) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation.
Recombinant human Fibroblast Growth Factor 8f (rhFGF-8f) produced in E. coli is a single non-glycosylated polypeptide chain containing 223 amino acids. A fully biologically active molecule, rhFGF-8f has a molecular mass of 25.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 50ng/mL, measured by a cell proliferation assayusing 3T3 cellsin the presence of 10 μg/ml of heparin, corresponding to a specific activity of > 2× 104 units/mg.|
MQEGPGRGPA LGRELASLFR AGREPQGVSQ QVTVQSSPNF
TQHVREQSLV TDQLSRRLIR TYQLYSRTSG KHVQVLANKR
INAMAEDGDP FAKLIVETDT FGSRVRVRGA ETGLYICMNK
KGKLIAKSNG KGKDCVFTEI VLENNYTALQ NAKYEGWYMA
FTRKGRPRKG SKTRQHQREV HFMKRLPRGH HTTEQSLRFE
FLNYPPFTRS LRGSQRTWAP EPR
|Measured Molecular Weight||25.5 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor 8f(rhFGF-8f) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-8f remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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