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Catalog Products » Recombinant Proteins » Growth Factors » Fibroblast Growth Factor-8f (FGF-8f), Human

FGF-8f, Human

Description Fibroblast Growth Factor 8f (FGF-8f) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation.
Recombinant human Fibroblast Growth Factor 8f (rhFGF-8f) produced in E. coli is a single non-glycosylated polypeptide chain containing 223 amino acids. A fully biologically active molecule, rhFGF-8f has a molecular mass of 25.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P55075
Species Human
Source E. coli
Biological Activity ED50 < 50ng/mL, measured by a cell proliferation assayusing 3T3 cellsin the presence of 10 μg/ml of heparin, corresponding to a specific activity of > 2× 104 units/mg.
Sequence
MQEGPGRGPA LGRELASLFR AGREPQGVSQ QVTVQSSPNF
TQHVREQSLV TDQLSRRLIR TYQLYSRTSG KHVQVLANKR
INAMAEDGDP FAKLIVETDT FGSRVRVRGA ETGLYICMNK
KGKLIAKSNG KGKDCVFTEI VLENNYTALQ NAKYEGWYMA
FTRKGRPRKG SKTRQHQREV HFMKRLPRGH HTTEQSLRFE
FLNYPPFTRS LRGSQRTWAP EPR

Measured Molecular Weight 25.5 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Fibroblast Growth Factor 8f(rhFGF-8f) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-8f remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z03203
Size
Price
10 ug $65.00
50 ug $159.00
1 mg $1500.00