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LAG-1/CCL4L1, Human

*This product has been discontinued!*
Recombinant human LAG-1/CCL4L1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhLAG-1/CCL4L1 has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Z03215
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Product Introduction
Species Human
Protein Construction
LAG-1 [Ala24-Asn92 (S70G)]
Accession # P13236
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity The EC50 value of human LAG-1 on Ca2+ mobilization assay in CHO-K1/Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells) is less than 50.0 ng/ml.
Expression System E. coli
Apparent Molecular Weight ~7.8 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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Background
Target Background Lymphocyte Activation Gene-1(Lag-1) , also known as C-C motif chemokine 4-like (CCL4L1), is a proinflammatory chemokine and is the isoform of Macrophage Inflammatory Protein-1 beta (MIP-1 beta). LAG-1 was first identified in 1988 and is expressed by monocytes, macrophages, T and B lymphocytes, and dendritic cells upon stimulation. The receptors of LAG-1 are the G-protein coupled receptors CCR1, CCR3, and CCR5 which is its major receptor. LAG-1 is a potent chemoattractant of monocytes, T lymphocytes, dendritic cells, natural killer cells, and activated CD4+ T cells. Unlike CCL4, LAG-1 cannot bind to glycosaminoglycans (GAGs), and thus cannot form an in vivo gradient on the cell surface as does CCL4. Like other members of the MIP-1 subfamily, LAG-1 is associated with several types of disease, including HIV infection and Type I diabetes.
Synonyms Lymphocyte Activation Gene-1; CCL4L1; ACT-2; T-cell activiation-2; HC21
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.