• English
  • Sign In
  • Contact Us
Catalog Products » Recombinant Proteins » Chemokines » LAG-1/CCL4L1, Human

LAG-1/CCL4L1, Human

Synonyms Lymphocyte Activation Gene-1, CCL4L1, ACT-2, T-cell activiation-2, HC21
Description Lymphocyte Activation Gene-1(Lag-1) , also known as C-C motif chemokine 4-like (CCL4L1), is a proinflammatory chemokine and is the isoform of Macrophage Inflammatory Protein-1 beta (MIP-1 beta). LAG-1 was first identified in 1988 and is expressed by monocytes, macrophages, T and B lymphocytes, and dendritic cells upon stimulation. The receptors of LAG-1 are the G-protein coupled receptors CCR1, CCR3, and CCR5 which is its major receptor. LAG-1 is a potent chemoattractant of monocytes, T lymphocytes, dendritic cells, natural killer cells, and activated CD4+ T cells. Unlike CCL4, LAG-1 cannot bind to glycosaminoglycans (GAGs), and thus cannot form an in vivo gradient on the cell surface as does CCL4. Like other members of the MIP-1 subfamily, LAG-1 is associated with several types of disease, including HIV infection and Type I diabetes.
Recombinant human LAG-1/CCL4L1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhLAG-1/CCL4L1 has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P13236
Source E. coli
Biological Activity The EC50 value of human LAG-1 on Ca2+ mobilization assay in CHO-K1/Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells) is less than 50 ng/ml.

Measured Molecular Weight 7.8 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Human LAG-1/CCL4L1 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human LAG-1/CCL4L1 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Note For research use only

Notice there is no results found, you can visit "Citations Database" for other results.

For more documents, please visit "Technical Support".

Cat. No. Z03215
10 ug $39.00
50 ug $99.00
1 mg $1350.00