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Catalog Products » Recombinant Proteins » Growth Factors » FGFR-1 alpha (IIIc)-Fc, Human

FGFR-1α (IIIc)-Fc, Human

Synonyms BFGFR, CD331
Description Fibroblast Growth Factor Receptor-1 (FGFR-1) is a transmembrane tyrosine kinase receptor belonging to the FGFR family. FGFR family has 4 members, FGFR-1 to FGFR-4, and they all have similar structural characteristics with 3 extracellular immunoglobulin-like (Ig) domains. FGFRs bind to FGFs with the second and third Ig domains, and complex with heparin sulfate when binding. The binding to FGF induces the dimerziation of FGFR and the phosphorylations of the intracellular tyrosines. Furthermore, the phosphorylated FGFR activates downstream signaling pathways, including STAT/JAK, RAS/MAPK, and PI3 K/AKT. Particularly, the signaling of FGFR-1 is stronger than that of FGFR-2, and sustains longer than that of FGFR-4. FGFR-1 is involved in the breast cancer: the patients with the FGFR-1 amplification are more likely to develop distant metastases, and the amplification of FGFR-1 is significantly associated with a shorter overall survival.
Recombinant human FGFR-1 alpha(IIIc)-Fc (rhFGFR-1 alpha(IIIc)-Fc) produced in Sf9 is a single glycosylated polypeptide chain containing 592 amino acids. A fully biologically active molecule, rhFGFR-1 alpha(IIIc)-Fc has a molecular mass of around 90 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P11362; P01857
Species Human
Source Sf9 insect cells
Biological Activity ED50 < 2 ng/mL, measured by the neutralization assay using 3T3 cells in presence of 4 ng/mL of human FGF-acidic, corresponding to a specific activity of > 5×105 units/mg.
Sequence
RPSPTLPEQA QPWGAPVEVE SFLVHPGDLL QLRCRLRDDV
QSINWLRDGV QLAESNRTRI TGEEVEVQDS VPADSGLYAC
VTSSPSGSDT TYFSVNVSDA LPSSEDDDDD DDSSSEEKET
DNTKPNPVAP YWTSPEKMEK KLHAVPAAKT VKFKCPSSGT
PNPTLRWLKN GKEFKPDHRI GGYKVRYATW SIIMDSVVPS
DKGNYTCIVE NEYGSINHTY QLDVVERSPH RPILQAGLPA
NKTVALGSNV EFMCKVYSDP QPHIQWLKHI EVNGSKIGPD
NLPYVQILKT AGVNTTDKEM EVLHLRNVSF EDAGEYTCLA
GNSIGLSHHS AWLTVLEALE ERPAVMTSPL YLEGSGSGSG
SPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR
TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ
YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT
ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS
DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS
RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

Measured Molecular Weight ~90 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human FGFR-1 alpha(IIIc)-Fc (rhFGFR-1 alpha(IIIc)-Fc) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGFR-1 alpha(IIIc)-Fc remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z03223
Size
Price
10 ug $39.00
50 ug $85.00
1 mg Quote