HVEM-Fc, Human
| Species | Human | ||||
| Protein Construction |
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| Purity |
> 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC |
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| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||||
| Biological Activity |
Assay #1: ED50 < 0.1 μg/ml, measured by the neutralization assay using 929 cells in presence of 0.25 ng/ml of human TNF-beta, corresponding to a specific activity of > 1.0 × 104 units/mg. Assay #2: Immobilized HVEM, hFc, Human at 2.0 μg/ml (100 µl/well) can bind biotinylated human BTLA. Assay #3: Immobilized HVEM, hFc, Human at 2.0 μg/ml (100 µl/well) can bind biotinylated CD160, hFc, Human (Cat. No.: Z03449). |
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| Expression System | Sf9 insect cells | ||||
| Apparent Molecular Weight | ~45 kDa, on SDS-PAGE under reducing conditions. | ||||
| Formulation | Lyophilized after extensive dialysis against PBS. | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background | Herpes Virus Entry Mediator (HVEM) is a transmembrane protein that is the receptor for TNFSF14 (also known as LIGHT) and is therefore referred to asTNFRSF14. HVEM is expressed broadly on immune cells such as T cells, natural killer (NK) cells and monocytes. The interaction of 3 molecules of LIGHT with three molecules of HVEM forms a hexameric complex that leads to the recruitment and retention of effector cells and activates NK cells to produce large amounts of IFN-γ and GM-CSF. In addition to the canonical binding partner LIGHT, HVEM can also bind to the inhibitory signaling protein, B- and T- lymphocyte attenuator (BTLA), which suppresses immune responses. Therefore, the HVEM network plays an important role in regulating immunity and the behavior of lymphocytes. |
| Synonyms | TNFRSF14; TR2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
For more documents, please visit "Technical Support".
| Cat. No. Z03224 | ||
|---|---|---|
| Size | Price | |
| 50 μg | $55.00 | |
| 1 mg | $465.00 | |
