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Catalog Products » Recombinant Proteins » Chemokines » LD78-beta/CCL3L1, Human

LD78-β/CCL3L1, Human

Synonyms SCYA3L1
Description LD78-beta/CCL3L1 is a proinflammatory chemokine and the isoform of Macrophage Inflammatory Protein-1 alpha (MIP-1 alpha). LD78-beta is secreted by most mature leukocytes, predominantly macrophages, and its major receptor is the G-protein coupled receptor CCR5, which is also the co-receptor used by the HIV-1 virus for cell entry. LD78-beta has superior antiviral activity and induces a variety of immune cells, particularly CD8+ T cells and immature dendritic cells. LD78-beta attracts lymphocytes and macrophages to sites of inflammation and infection, and its functions are inhibited by Interleukin-4, Interleukin-10, and Interleukin-13. Importantly, the copy number variation of LD78-beta is associated with HIV susceptibility, indicating LD78-beta’s critical role in the disease.
Recombinant human LD78-beta/CCL3L1 (rhLD78-beta) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhLD78-beta has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P16619
Species Human
Source E. coli
Biological Activity ED50< 0.4 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3L1, corresponding to a specific activity of > 2.5×103 units/mg.
Sequence
APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV
IFLTKRGRQV CADPSEEWVQ KYVSDLELSA

Measured Molecular Weight 7.8 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human LD78-beta/CCL3L1 (rhLD78-beta) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhLD78-beta remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z03226
Size
Price
5 ug $40.00
25 ug $95.00
1 mg $1500.00