LD78-β/CCL3L1, Human
| Synonyms | SCYA3L1 |
| Description | LD78-beta/CCL3L1 is a proinflammatory chemokine and the isoform of Macrophage Inflammatory Protein-1 alpha (MIP-1 alpha). LD78-beta is secreted by most mature leukocytes, predominantly macrophages, and its major receptor is the G-protein coupled receptor CCR5, which is also the co-receptor used by the HIV-1 virus for cell entry. LD78-beta has superior antiviral activity and induces a variety of immune cells, particularly CD8+ T cells and immature dendritic cells. LD78-beta attracts lymphocytes and macrophages to sites of inflammation and infection, and its functions are inhibited by Interleukin-4, Interleukin-10, and Interleukin-13. Importantly, the copy number variation of LD78-beta is associated with HIV susceptibility, indicating LD78-beta’s critical role in the disease. Recombinant human LD78-beta/CCL3L1 (rhLD78-beta) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhLD78-beta has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Accession No | P16619 |
| Species | Human |
| Source | E. coli |
| Biological Activity | ED50< 0.4 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3L1, corresponding to a specific activity of > 2.5×103 units/mg. |
| Sequence | APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA |
| Measured Molecular Weight | 7.8 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE and HPLC analysis. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/mL. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human LD78-beta/CCL3L1 (rhLD78-beta) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhLD78-beta remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |
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| Cat. No. Z03226 | ||
|---|---|---|
Size |
Price |
|
| 5 ug | $40.00 | |
| 25 ug | $95.00 | |
| 1 mg | $1500.00 | |
