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Catalog Products » Recombinant Proteins » Growth Factors » Hepatocyte Growth Factor (HGF), Human

HGF, Human

Description Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.
Recombinant human Hepatocyte Growth Factor (rhHGF) is produced in CHO cells and consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in the formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. A fully biologically active molecule, rhHGF has a molecular mass of around 88-90 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P14210
Species Human
Source CHO
Biological Activity ED50 < 10 ng/ml, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1×105units/mg.
Sequence
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA
NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE
FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP
WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT
SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI
CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC
YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE
GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE
NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC
YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD
ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG
DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL
RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH
DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD
DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV
AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG
DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY
YAKWIHKIIL TYKVPQS

Measured Molecular Weight 88-90 kDa (single chain), 59-61kDa (alpha chain), 30-34kDa (beta chain), observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant humanHepatocyte Growth Factor (HGF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human Hepatocyte Growth Factor (HGF) should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Note For research use only




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Cat. No. Z03229
Size
Price
10 ug $85.00
50 ug $249.00
1 mg $1900.00