|Synonyms||Serpin B5, PI-5|
|Description||Maspin is a non-inhibitory member of the serpin superfamily, a group of 40-60 kDa serine protease inhibitors. Maspin was discovered in 1994 and has been identified in prostate, thymus, testis, intestine, tongue, and lung. Recently Maspin received a great deal of attention due to its potential apoptotic role in cancers. Maspin expression predicts a better prognosis for several types of carcinomas including breast, prostate, colon, and oral squamus cell carcinoma. The level of Maspin is also inversely correlated to VEGF-A, an angiogenic factor. Researchers found that Maspin may play its apoptotic role as an endogenous inhibitor of histone deacetylase I (HDAC I). Therefore, Maspin is a potential personalized marker of Non-Small Cell Lung Carcinoma (NSCLC).
Recombinant human Maspin (rhMaspin) produced in E. coli is a single non-glycosylated polypeptide chain containing 375 amino acids. rhMaspin has a molecular mass of 42.1 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Data not available.|
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS
LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL
SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD
FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL
VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM
EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES
TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK
MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH
KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH
|Measured Molecular Weight||42.1 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analysis.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Maspin (rhMaspin) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhMaspin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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