|Synonyms||Ckbeta 8‑1/CCL23, Human|
|Description||Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family that is also known as Macrophage inflammatory protein 3 (MIP-3) and Myeloid progenitor inhibitory factor 1 (MPIF-1). CCL23 is predominantly expressed in lung and liver tissue, but is also found in bone marrow and placenta.CCL23 is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells. CCL23 is a ligand for the chemokine receptor CCR1.
Recombinant human Ck beta 8‑1/CCL23 produced in CHO cells is a single polypeptide chain containing 92 amino acids. A fully biologically active molecule, rhCk beta 8‑1/CCL23 has a molecular mass of 10.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||The EC50 value of human Ck beta 8‑1/CCL23 on Ca2+ mobilization assay in CHO-K1/Ga15/hCCR1 cells (human Ga15 and human CCR1 stably expressed in CHO-K1 cells) is less than 0.4 μg/ml.|
MLWRRKIGPQ MTLSHAAGFH ATSADCCISY TPRSIPCSLL
ESYFETNSEC SKPGVIFLTK KGRRFCANPS DKQVQVCVRM
|Measured Molecular Weight||10.5 kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Ck beta 8‑1/CCL23 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human Ck beta 8‑1/CCL23 should be stable up to 1 week at 4°C or up to 2 months at -20°C.|
|Note||For research use only|
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