• English
  • Sign In
  • Contact Us
×
Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » Granzyme B, Mouse

Granzyme B, Mouse

Synonyms Granzyme B, Mouse
Description Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs), natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells.
Granzyme B has also recently been found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The secondary functions of granzyme B are also numerous. Granzyme B has been shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling.
Recombinant Mouse Granzyme B produced in CHO cells is a polypeptide chain containing 227 amino acids. A fully biologically active molecule, rmGranzyme B has a molecular mass of 32 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P04187
Species Mouse
Source CHO
Biological Activity Measured by its ability to cleave a chromogenic peptide substrate (Ac-IEPD-pNA), the specific activity is greater than 1000 nM/min per μg of enzyme.
Sequence
IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL
TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY
NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD
VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR
YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG
YKDGSPPRAF TKVSSFLSWI KKTMKSS

Measured Molecular Weight 32 kDa, observed by reducing SDS-PAGE.
Purity > 98% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Mouse Granzyme B remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, recombinant Mouse Granzyme B should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Note For research use only




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. Z03239
Size
Price
10 ug $89.00
50 ug $410.00
1 mg $3500.00