|Synonyms||Granzyme B, Mouse|
|Description||Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs), natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells.
Granzyme B has also recently been found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The secondary functions of granzyme B are also numerous. Granzyme B has been shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling.
Recombinant Mouse Granzyme B produced in CHO cells is a polypeptide chain containing 227 amino acids. A fully biologically active molecule, rmGranzyme B has a molecular mass of 32 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Measured by its ability to cleave a chromogenic peptide substrate (Ac-IEPD-pNA), the specific activity is greater than 1000 nM/min per μg of enzyme.|
IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL
TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY
NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD
VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR
YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG
YKDGSPPRAF TKVSSFLSWI KKTMKSS
|Measured Molecular Weight||32 kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Mouse Granzyme B remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, recombinant Mouse Granzyme B should be stable up to 1 week at 4°C or up to 2 months at -20°C.|
|Note||For research use only|
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