|Synonyms||C-X-C motif chemokine 5, Small-inducible cytokine B5, Cytokine LIX, Cxcl5, Scyb5, LIX, GCP-2, Scyb6, ENA-78, AMCF-II.|
|Description||Mouse LIX (C-X-C motif chemokine 5) is a small cytokine belonging to the CXC chemokine family that is cleaved into the following 2 chains [GCP-2(1-78) and GCP-2(9-78)]. Mouse LIX plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which could be used to understand more about pain in other inflammatory conditions. It is most closely related to two highly homologous human neutrophil chemoattractants GCP-2 and ENA-78. The first 78 amino acid residues within the predicted mature mouse LIX shares approximately 61% and 55% amino acid identity with human GCP-2 and ENA-78. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.
Recombinant mouse LIX/CXCL5 (74aa) produced in CHO cells is a single polypeptide chain containing 74 amino acids. rmLIX/CXCL5(74aa) has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||The EC50 value of mouse LIX/CXCL5 (74aa) on Ca2+ mobilization assay in CHO-K1/Gα15/mCXCR2 cells (human Gα15 and mouse CXCR2 stably expressed in CHO-K1 cells) is less than 200 ng/mL.|
VIAATELRCV CLTVTPKINP KLIANLEVIP AGPQCPTVEV
IAKLKNQKEV CLDPEAPVIK KIIQKILGSD KKKA
|Measured Molecular Weight||8 kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse LIX/CXCL5 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution,mouse LIX/CXCL5 should be stable up to 1 week at 4°C or up to 2 months at -20°C.|
|Note||For research use only|
For more documents, please visit "Technical Support".