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Catalog Products » Recombinant Proteins » Growth Factors » Macrophage Colony Stimulating Factor(M-CSF), Mouse 

M-CSF, Mouse 

Synonyms Macrophage Colony Stimulating Factor, CSF-1, MGI-IM, M-CSF
Description Macrophage Colony Stimulating Factor (M-CSF) ,also known as CSF1,is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells.M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity.M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.
Recombinant mouse Macrophage Colony Stimulating Factor (M-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 156 amino acids each. A fully biologically active molecule, rmM-CSF has a molecular mass of 30 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P07141
Species Mouse
Source E. coli
Biological Activity ED50< 3 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 3.3×105 units/mg.
Sequence
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD
QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER
LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN
FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Measured Molecular Weight 30 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against 50mMTris,150mMNaCl, pH 8.0.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Mouse Macrophage Colony Stimulating Factor(M-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse M-CSF should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Note For research use only




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Cat. No. Z03275
Size
Price
10 ug $85.00
50 ug $275.00