|Synonyms||Heparin Binding EGF-like growth factor, HBEGF, Diphtheria toxin receptor, DTR|
|Description||Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds to the EGF receptor with a higher affinity and has been shown to bemore mitogenic, likely due to its ability to bind to heparin and heparin sulfate proteoglycans. HB-EGF has also been reported to act as a diphtheria toxin receptor, mediating endocytosis of the bound toxin. Heparin-binding EGF-like growth factor has been shown to interact with NRD1, Zinc finger and BTB domain-containing protein 16 and BAG1.
Recombinant Human HB-EGF produced in E. coli cells is a polypeptide chain containing 86 amino acids. A fully biologically active molecule, rhHB-EGF has a molecular mass of 14 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.75ng/ml, measured in a cell proliferation assay using 3T3 cells.|
Asp63-Leu148 (Accession #:Q99075)
|Measured Molecular Weight||14 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human Proheparin-binding EGF-like Growth Factor (HB-EGF), remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human HB-EGF should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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