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Catalog Products » Recombinant Proteins » Growth Factors » Proheparin-binding EGF-like Growth Factor (HB-EGF), Human

HB-EGF, Human

Synonyms Heparin Binding EGF-like growth factor, HBEGF, Diphtheria toxin receptor, DTR
Description Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds to the EGF receptor with a higher affinity and has been shown to bemore mitogenic, likely due to its ability to bind to heparin and heparin sulfate proteoglycans. HB-EGF has also been reported to act as a diphtheria toxin receptor, mediating endocytosis of the bound toxin. Heparin-binding EGF-like growth factor has been shown to interact with NRD1, Zinc finger and BTB domain-containing protein 16 and BAG1.
Recombinant Human HB-EGF produced in E. coli cells is a polypeptide chain containing 86 amino acids. A fully biologically active molecule, rhHB-EGF has a molecular mass of 14 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No Q99075
Source E. coli
Biological Activity ED50 < 0.75ng/ml, measured in a cell proliferation assay using 3T3 cells.
Sequence
Asp63-Leu148 (Accession #:Q99075)

Measured Molecular Weight 14 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Human Proheparin-binding EGF-like Growth Factor (HB-EGF), remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human HB-EGF should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Note For research use only


Afonin KA., et al. Multifunctional RNA Nanoparticles. Nano Lett. 2014July;14(10):5662-71

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Cat. No. Z03291
Size
Price
10 ug $40.00
50 ug $90.00
1 mg $750.00