|Synonyms||Fibroblast Growth Factor-8, FGF-8e, AIGF, HBGF-8|
|Description||Fibroblast Growth Factor 8e (FGF-8e) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation.
Recombinant Human FGF-8e produced in E. coli is a single non-glycosylated polypeptide chain containing 212 amino acids. A fully biologically active molecule, rhFGF-8e has a molecular mass of 24.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 2.5µg/ml in the presence of 1 µg/mL heparin, measured in a cell proliferation assay using 3T3.|
Gln23-Arg233 (Accession #:P55075), with an N-terminal Met
|Measured Molecular Weight||24.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human FGF 8e remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human FGF 8e should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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