CNTF, Rat

Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone which acts within the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF is a potent survival factor for neurons and oligodendrocytes and may play a role in reducing tissue damage during increased inflammation. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, however this phenotype is not causally related to neurologic disease.

Price	$74.00
Cat. No.	Z03313-10
Size	10 μg 50 μg 1 mg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Rat

Protein Construction

CNTF (Ala2-Met200)
Accession # P20294-1

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 30.0 ng/ml, measured by its ability to induce alkaline phosphatase production by TF-1 Cells.

Expression System

E. coli

Apparent Molecular Weight

~22.9 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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Background

Target Background

Synonyms

Ciliary Neurotrophic Factor

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.