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Catalog Products » Recombinant Proteins » Cytokines » Tumor Necrosis Factor-alpha (TNF-α),Bovine

TNF-α, Bovine

Synonyms Bovine TNF-α
Description Tumor Necrosis Factor-Alpha (TNF-α) plays a major role in regulating growth, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. In addition to inducing hemorrhagic necrosis of tumors, studies indicate TNF is involved in tumor igenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, Crohn's disease, rheumatoid arthritis and graft-versus-host disease.
Recombinant Bovine Tumor Necrosis Factor-alpha (rbTNF-α) produced in E. coli cells is a single non-glycosylated polypeptide chain containing 80 amino acids. A fully biologically active molecule, rbTNF-α has a molecular mass of 17.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No Q06599
Source E. coli
Biological Activity ED50 < 0.1 µg/ml, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 1 × 104 units/mg.
Sequence
MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN
GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT
KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP
DYLDYAESGQ VYFGIIAL

Measured Molecular Weight 17.6 kDa, observed by reducing SDS-PAGE
Purity > 95% as analyzed by SDS-PAGE & HPLC
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Bovine TNF-α remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Bovine TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Note For research use only




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Cat. No. Z03316
Size
Price
50 ug $160.00
10 ug $60.00
1 mg $1350.00