|Synonyms||Human Cardiac Troponin I|
|Description||Cardiac Troponin I (cTnI) is a subtype of the troponin family that is commonly used as a marker for myocardial damage. Cardiac troponin I is specific for cardiac tissue and is detected in the serum only if myocardial injury has occurred. Because cardiac troponin I is a very sensitive and specific indicator of heart muscle (myocardium) damage, serum levels can be used to help differentiate between unstable angina and myocardial infarction (heart attack) in people with chest pain or acute coronary syndrome.
Recombinant Human Cardiac Troponin I (cTnI) produced in E. coli cells is a single non-glycosylated polypeptide chain containing 222 amino acids. rhcTnI has a molecular mass of 26 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Not Available|
MHHHHHHDDD DKMADGSSDA AREPRPAPAP IRRRSSNYRA
YATEPHAKKK SKISASRKLQ LKTLLLQIAK QELEREAEER
RGEKGRALST RCQPLELAGL GFAELQDLCR QLHARVDKVD
EERYDIEAKV TKNITEIADL TQKIFDLRGKFKRPTLRRVR ISADAMMQAL
LGARAKESLD LRAHLKQVKK EDTEKENREV GDWRKNIDAL
|Measured Molecular Weight||26 kDa, observed by reducing SDS-PAGE|
|Purity||> 95% as analyzed by SDS-PAGE|
|Formulation||Lyophilized after extensive dialysis against 10 mM HCl.|
|Reconstitution||Reconstitute with Tris/ urea buffer, 20 mM Tris, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol, pH 7.5.|
|Endotoxin Level||Not Available|
|Storage||Lyophilized recombinant Human cTnI remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human cTnI should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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