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Catalog Products » Recombinant Proteins » Other Catalog Proteins » Cathepsin L, Human

Cathepsin L, Human

Synonyms Cathepsin L; CTSL; CTSL1
Description Cathepsin L, also known as Aldrichina grahami cysteine proteinase, is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has also been shown to proteolytically inactivate α-1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract. Cathepsin L expression has been reported in many organisms including fish, birds and mammals.
Recombinant Human Cathepsin L produced in CHO cells is a polypeptide chain containing 227 amino acids. A fully biologically active molecule, rhCathepsin L has a molecular mass of 40 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P07711
Source CHO
Biological Activity The Specific Activity is > 2000 pmol/min/μg, measured by Cathepsin L’s ability to cleave the fluorogenic peptide substrate Z-FR-AMC (Enzo, Catalog: P139).
Assay buffer: 400 mM sodium acetate, pH5.5,4 mM EDTA, 8 mM DTT
Sequence
EAPRSVDWRE KGYVTPVKNQ GQCGSCWAFS ATGALEGQMF
RKTGRLISLS EQNLVDCSGP QGNEGCNGGL MDYAFQYVQD
NGGLDSEESY PYEATEESCK YNPKYSVAND TGFVDIPKQE
KALMKAVATV GPISVAIDAG HESFLFYKEG IYFEPDCSSE
DMDHGVLVVG YGFESTESDN NKYWLVKNSW GEEWGMGGYV
KMAKDRRNHC GIASAASYPT VHHHHHH

Measured Molecular Weight 40 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE & HPLC.
Formulation Liquid after a 0.2 μm filtered solution in 50 mM NaOAc, 50 mM NaCl, 20% Glycerol, pH 6.0.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Recombinant Human Cathepsin L remains stable up to 6 months at lower than -70°C from date of receipt under sterile conditions. Up to 3 months at lower than -70°C under sterile conditions after opening. Avoid repeated freeze-thaw cycles.
Note For research use only




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Cat. No. Z03323
Size
Price
10 ug $145.00