|Description||Interleukin-17 receptor D (IL-17RD) , also known as similar expression to the fibroblast growth factor receptor (SEF), is a member of the interleukin-17 receptor family. Studies indicate that IL-17RD interacts with IL-17R to facilitate IL-17 signaling. Additionally, IL-17RD may play an important role in preventing certain types of cancer. Studies suggest that IL-17D acts as a feedback inhibitor of fibroblast growth factor-mediated MAPK signaling and ERK activation. Studies indicate that IL-17D can bind to MEK/ERK complexes preventing nuclear ERK translocation and subsequent cell proliferation.
Recombinant Mouse IL-17 RD produced in CHO cells is a polypeptide chain containing 271 amino acids. A fully biologically active molecule, rmIL-17 RD has a molecular mass of 53-56 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Specific Activity||ED50 < 5 μg /ml, measured in a cell proliferation assay using wt MEF cells.|
GSGRARGADT CGWRGVGPAS RNSGLHNITF RYDNCTTYLN
PGGKHAIADA QNITISQYAC HDQVAVTILWSPGALGIEFL KGFRVILEEL
KSEGRQCQQL ILKDPKQLNS SFRRTGMESQ PFLNMKFETD
YFVKIVPFPSIKNESNYHPF FFRTRACDLL LQPDNLACKP FWKPRNLNIS
QHGSDMHVSF DHAPQNFGFR GFHVLYKLKHEGPFRRRTCR QDQNTETTSC
LLQNVSPGDY IIELVDDSNT TRKAAQYVVK SVQSPWAGPI
|Measured Molecular Weight||53-56 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE&HPLC.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Mouse Interleukin-17 D Receptor remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse Interleukin-17 D Receptor should be stable up to 1 week at 4°C or up to 2 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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