|Synonyms||Human TGF-α,Human TGF-type I|
|Description||Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. It is expressed in monocytes, brain cells, keratinocytes and various tumor cells. TGF-α signals through EGFR and acts synergistically with TGF-beta to promote the proliferation of a wide range of epidermal and epithelial cells. TGF-α is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. The biological actions of TGF-α and EGF are similar. For instance, TGF-α and EGF bind to the same receptor. When TGF-α binds to EGFR it can initiate multiple cell proliferation events.
Recombinant Human TGF-α produced in E. coli cells is a single non-glycosylated polypeptide chain containing 50 amino acids. A fully biologically active molecule, rhTGF-α has a molecular mass of 5.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.2ng/ml, measured by Cell Proliferation Assay using BALB/c 3T3 cells, corresponding to a specific activity of ≥ 5 x 106 units/mg.|
VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG
|Measured Molecular Weight||5.8 kDa, observed by reducing SDS-PAGE|
|Purity||> 95% as analyzed by SDS-PAGE|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human TGF-α remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human TGF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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