|Synonyms||TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2|
|Description||Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseasesuch as Crohn's disease, rheumatoid arthritis and graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Recombinant MouseTNF-α(80-235aa) produced in E. coli is a singlenon-glycosylated polypeptidechain containing 156 amino acids. A fully biologically active molecule, rmTNF-α (80-235aa) has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Specific Activity||ED50 < 0.05 ng/mL, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >2 x 10 7 units/mg.|
LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG
MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS
RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG
GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIAL
|Measured Molecular Weight||17.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by SDS-PAGE&HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant MouseTNF-α (80-235aa) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse TNF-α (80-235 aa) should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
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