|Synonyms||Human UBCH10; Human UBE2C; Ubiquitin-conjugating enzyme E2 C|
|Description||Ubiquitinconjugating Enzyme E2 C(UBE2C), known as Ubiquitinconjugating Enzyme H10(UbcH10), is a 179 amino acid(aa) member of the yeast Ubc4/5 family of Ubiquitinconjugating(E2) enzymes and has a predicted molecular weight of 19.5 kDa. UbcH10/UBE2C plays a critical role in the destruction of mitotic proteins which is essential for subsequent events in cell cycle progression. UbcH10/UBE2C recognizes TEK sequences in target proteins, mediates Lys11linked ubiquitination, and helps promote targeted protein degradation.
Recombinant Human UBE2C produced in E. coli cells is a single non-glycosylated polypeptide chain containing 179 amino acids. A fully biologically active molecule, rhUBE2C has a molecular mass of 19.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Not Available|
MASQNRDPAA TSVAAARKGA EPSGGAARGP VGKRLQQELM
TLMMSGDKGI SAFPESDNLF KWVGTIHGAA GTVYEDLRYK
LSLEFPSGYP YNAPTVKFLT PCYHPNVDTQ GNICLDILKE
KWSALYDVRT ILLSIQSLLG EPNIDSPLNT HAAELWKNPT
|Measured Molecular Weight||19.5 kDa, observed by reducing SDS-PAGE|
|Purity||> 95% as analyzed by SDS-PAGE&HPLC|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human UBE2C remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human UBE2C should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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