|Synonyms||Fibroblast Growth Factor-17, FGFH|
|Description||Fibroblast Growth Factor-17 (FGF-17) is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play multiple roles in biological functions, including angiogenesis, mitogenesis, cell differentiation and wound repair. FGF-17 plays an important role in organizing and inducing specific patterning at the midbrain/hindbrain junction. FGF-17 is also expressed in the hindgut, parts of thedeveloping skeleton, tail bud, major arteries, and heart. FGF-17 signals through hFGFR1c, 2c, 3c, and 4. FGF-17 signals induce patterning of the embryonic brain.
Recombinant Human FGF-17 produced in E .coli is a single non-glycosylated polypeptide chain containing 195 amino acids. A fully biologically active molecule rhFGF-17 has a molecular mass of 22.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5 ng/mL, measured by the cell proliferation assay using Balb/c3T3 cells, corresponding to a specific activity of >2 × 106 units/mg.|
MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT
SGKHVQVTGR RISATAEDGNKFAKLIVETD TFGSRVRIKG AESEKYICMN
KRGKLIGKPS GKSKDCVFTE IVLENNYTAFQNARHEGWFM AFTRQGRPRQ
ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSAPTRRTKRTRR
|Measured Molecular Weight||22.6 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human Fibroblast Growth Factor-17 (FGF-17) remainsst able up to 6 months at lower than -70°C from date of receipt. Upon reconstitution Human FGF-17 should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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