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Catalog Products » Recombinant Proteins » Other Catalog Proteins » UBE2B/HR6B, Human

UBE2B/HR6B, Human

Synonyms Ubiquitin-conjugating enzyme E2 B, EC 6.3.2.19, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa UBC2, HHR6B, RAD6B, E2-17kDa, UBE2B.
Description Ubiquitin­conjugating Enzyme E2 B (UBE2B), known as Homolog of Rad6B (HR6B), is a member of the Ubiquitin­conjugating (E2) enzyme family. It is ubiquitously expressed with a predicted molecular weight of 17 kDa. HR6B/UBE2B localizes to the nucleus and cytoplasm and has an E2 catalytic core domain that forms a thioester bond with Ubiquitin. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multi-ubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.
Recombinant Human UBE2B produced in E. coli cells is a single non-glycosylated polypeptide chain ontaining 152 amino acids. A fully biologically active molecule, rhUBE2B has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P63146
Source E. coli
Biological Activity Not Available.
Sequence
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI
FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN
VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

Measured Molecular Weight 17.3 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE&HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Human UBE2B/HR6B remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human UBE2B/HR6B should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Note For research use only


Cat. No.
Product Name
Price
Z03334-10
UBE2C/UBCH10, Human - 10 ug
$39.00
 


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Cat. No. Z03340
Size
Price
10 ug $39.00
50 ug $69.00