|Synonyms||Ubiquitin-conjugating enzyme E2 B, EC 188.8.131.52, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa UBC2, HHR6B, RAD6B, E2-17kDa, UBE2B.|
|Description||Ubiquitinconjugating Enzyme E2 B (UBE2B), known as Homolog of Rad6B (HR6B), is a member of the Ubiquitinconjugating (E2) enzyme family. It is ubiquitously expressed with a predicted molecular weight of 17 kDa. HR6B/UBE2B localizes to the nucleus and cytoplasm and has an E2 catalytic core domain that forms a thioester bond with Ubiquitin. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multi-ubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.
Recombinant Human UBE2B produced in E. coli cells is a single non-glycosylated polypeptide chain ontaining 152 amino acids. A fully biologically active molecule, rhUBE2B has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Not Available.|
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI
FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN
VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS
|Measured Molecular Weight||17.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE&HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human UBE2B/HR6B remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human UBE2B/HR6B should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
|Note||For research use only|
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