|Description||Kallikrein 6 (KLK-6) , also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis, and some have potential as novel cancer and other disease biomarkers. KLK-6 was originally characterized from the brain as an enzyme involved in the degradation of amyloid plaque protein (APP) and was thought to be a beta secretase. KLK-6 was shown to be elevated in the sera of patients with Alzheimer’s disease, Parkinson’s disease and in animal models of multiple sclerosis. Studies indicate KLK-6 may participate in the demyelination processes and progression of CNS inflammatory disease.
Recombinant Human Kallikrein 6 produced in CHO cells is a polypeptide chain containing 238 amino acids. A fully biologically active molecule, rhKLK6 has a molecular mass of 31 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||The Specific Activity is > 1200 pmol/min/μg, Measured by hKLK6’s ability to cleave the fluorogenic peptide substrate Boc-Gln-Ala-Arg-AMC (R&D, ES014)
Activation Buffer: 50 mM Tris, 0.05% (w/v) Brij35, pH 8.0
Assay Buffer: 50 mM Tris, 1.0 M sodium Citrate, pH 7.5
Dilute hKLK6 to 200 ug/ml in Activation Buffer
Dilute lysyl endpeptidase to 2.5 mU/mL in Activation Buffer.
Combine equal volumes of 200 ug/ml hKLK6 and 2.5mU/ml lysyl-endpeptidase
Incubate at room temperature for 30 minutes.
EEQNKLVHGG PCDKTSHPYQ AALYTSGHLL CGGVLIHPLW
VLTAAHCKKP NLQVFLGKHN LRQRESSQEQ SSVVRAVIHP DYDAASHDQD
IMLLRLARPA KLSELIQPLP LERDCSANTT SCHILGWGKT ADGDFPDTIQ
CAYIHLVSRE ECEHAYPGQI TQNMLCAGDE KYGKDSCQGD SGGPLVCGDH
LRGLVSWGNI PCGSKEKPGV YTNVCRYTNW IQKTIQAKHH
|Measured Molecular Weight||31 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Liquid after a 0.2 μm filtered solution in 25 mM NaOAc, 50 mM NaCl, pH 6.0.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Recombinant Human Kallikrein 6 remains stable up to 6 months at lower than -70°C from date of receipt under sterile conditions. Up to 3 months at lower than -70°C under sterile conditions after opening. Avoid repeated freeze-thaw cycles.|
|Note||For research use only|
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