|Synonyms||PD-1; CD279; PDCD1|
|Description||Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279) or PDCD1, is a protein that in humans is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells.PD-1 binds two ligands, PD-L1 and PD-L2. PD-1 and its ligands play an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells)
Recombinant Human PD-1 produced in CHO cells with C-terminal Fc tag, is a polypeptide chain containing 378 amino acids. A fully biologically active molecule, rhPD-1 has a molecular mass of 60-65 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Specific Activity||Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1/B7-H1 Fc Chimera at 1 μg/ml (100 μl/well) can bind biotinylated rhPD-1 Fc Chimera with a linear range of 0.1-1 μg/ml when detected by HRP*-Labeled Streptavidin.|
LDSPDRPWNP PTFSPALLVV TEGDNATFTC SFSNTSESFV
LNWYRMSPSN QTDKLAAFPE DRSQPGQDCR
FRVTQLPNGR DFHMSVVRAR RNDSGTYLCG
AISLAPKAQI KESLRAELRV TERRAEVPTA HPSPSPRPAG
|Measured Molecular Weight||60-65kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human PD-1 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human PD-1 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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