|Synonyms||PD-L1; CD274; PDCD1L1; PDCD1LG1; B7-H1|
|Description||Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40 kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition.
Recombinant Human PD-L1(B7-H1) Fc Chimera produced in CHO cells is a polypeptide chain containing 457 amino acids. A fully biologically active molecule, rh PD‑L1(B7-H1) has a molecular mass of 70-72 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Activity1: Immobilized PD-L1/B7-H1 Fc Chimera, Human at 1 μg/ml (100 μl/well) can bind biotinylated PD-1 Fc Chimera(Cat.No.Z03370) with a linear range of 0.1-1 μg/ml.
Acitivity2: Immobilized PD-1, His, Human(Cat.No.Z03424) at 2 μg/mL can bind PD-L1 Fc Chimera, Human with a linear range of 24-390ng/mL.
FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE
MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA
LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN
QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK
TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE
NHTAELVIPE LPLAHPPNER T
|Measured Molecular Weight||70-72kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human PD‑L1(B7-H1) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human PD‑L1(B7-H1) should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
|Note||For research use only|
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