CTLA-4 Fc Chimera, Human
| Synonyms | CTLA-4 |
| Description | Cytotoxic T lymphocyte-associated molecule-4 (CTLA-4) is a cell surface molecule that is closely related to CD28, and a powerful negative regulator of T cell activation. Structurally, CTLA-4 is a member of the Ig superfamily, having a single extracellular V-like domain , homology with CD28; The overall sequence homology between CD28 and CTLA-4 is about 20%, but they share a 27%(murine) to 31%(human) identity at the amino acid level. Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Recombinant Human CTLA-4 Fc Chimera produced in CHO cells is a polypeptide chain containing 378 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhCTLA-4 has a molecular mass of 45-48 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Species | Human |
| Source | CHO |
| Biological Activity | Activity1: Measured by its ability to inhibit IL-2 secretion by co-culturing stimulated Jurkat human acute T cell leukemia cells and CD80 expression CHO stable cell line. Activity2:Immobilized B7-2(CD86), His, Human(Cat.No.Z03452) at 2 μg/mL (100 μL/well) can bind CTLA-4 Fc Chimera, Human with a linear range of 0.97-7.8 ng/mL |
| Sequence | AMHVAQPAVV LASSRGIASF VCEYASPGKA TEVRVTVLRQ ADSQVTEVCA ATYMMGNELT FLDDSICTGT SSGNQVNLTI QGLRAMDTGL YICKVELMYP PPYYLGIGNG TQIYVIDPEP CPDSDF |
| Measured Molecular Weight | 45-48 kDa, observed by reducing SDS-PAGE. |
| Purity | > 98% as analyzed by reducing SDS-PAGE. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | <0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant Human CTLA-4 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution Human CTLA-4 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
GenScript product showed equal activity compared to competitor A when binding with biotin CD80 Fc,Human.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01833-40 |
Human CTLA-4 Antibody (CT.E8), mAb, Mouse - 40 ug |
$99.00 |
|
A01832-40 |
Human CTLA-4 Antibody (CT.F3), mAb, Mouse - 40 ug |
$99.00 |
For more documents, please visit "Technical Support".
| Cat. No. Z03373 | ||
|---|---|---|
| Size | Price | |
| 50 ug | $65.00 | |
| 1 mg | $650.00 | |
