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CTLA-4 Fc Chimera, Mouse

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152, is an Inhibitory receptor acting as a major negative regulator of T-cell responses. CTLA-4 is a member of the immunoglobulin superfamily, which is expressed on the surface of T cells and transmits an inhibitory signal to T cells. CTLA-4 and CD28 are homologous receptors expressed by both CD4+ and CD8+ T cells, which mediate opposing functions in T-cell activation. Both receptors share a pair of ligands expressed on the surface of antigen-presenting cells (APCs). The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory co-receptor CD28.
$58.00
Z03391-50

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Product Introduction
Species Mouse
Protein Construction
CTLA-4 (Ala37-Phe162)
Accession # Q6GTR6		 mFc
N-term C-term
Purity > 90% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity Immobilized Mouse B7-1/CD80 Protein, His Tag at 2.0 μg/ml can bind CTLA-4, mFc, Mouse with EC50=12.57 ng/ml when detected by M6 Goat Anti Mouse FC.
Expression System CHO
Apparent Molecular Weight ~54.3 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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CTLA-4 Fc Chimera, Mouse

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CTLA-4 Fc Chimera, Mouse

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CTLA-4 Fc Chimera, Mouse

Mouse B7-1/CD80 Protein, His Tag at 2 μg/ml (100 μl/well) can bind CTLA-4 Fc Chimera, Mouse with EC50=12.57 ng/ml when detected by M6 Goat Anti Mouse FC. Background was subtracted from data points before curve fitting. »

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Background
Target Background CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152, is an Inhibitory receptor acting as a major negative regulator of T-cell responses. CTLA-4 is a member of the immunoglobulin superfamily, which is expressed on the surface of T cells and transmits an inhibitory signal to T cells. CTLA-4 and CD28 are homologous receptors expressed by both CD4+ and CD8+ T cells, which mediate opposing functions in T-cell activation. Both receptors share a pair of ligands expressed on the surface of antigen-presenting cells (APCs). The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory co-receptor CD28.
Synonyms CD152
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.