|Synonyms||Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3|
|Description||TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules that a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that co-express PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens.
Recombinant Mouse TIM-3 Fc Chimera produced in HEK293 cells is a polypeptide chain containing 406 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rmTIM-3 has a molecular mass of 62 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Measured by its binding ability in a functional ELISA. Immobilized human Galectin at 0.5 μg/mL (100 μL/well) can bind TIM-3 Fc, Mouse(Cat.No.Z03401) with a linear range of 0.78-6.25 μg/mL. Background was subtracted from data points before curve fitting.|
Leu²²-Ala¹⁹³ (Accession #: Q8VIM0-1), expressed with a C-terminal human IgG1 Fc fragment.
LENAYVFE VGKNAYLP CSYTLSTP GALVPMCW GKGFCPWS
QCTNELLR TDERNVTY QKSSRYQL KGDLNKGD VSLIIKNV
TLDDHGTY CCRIQFPG LMNDKKLE LKLDIKAA KVTPAQTA
HGDSTTAS PRTLTTER NGSETQTL VTLHNNNG TKISTWAD
|Molecular Weight||62 kDa, observed by reducing SDS-PAGE.|
|Purity||> 97% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant TIM-3 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse TIM-3 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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