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Home » Gene Synthesis & Molecular Biology Services » TIM-3 Fc Chimera, Mouse

TIM-3 Fc Chimera, Mouse

Synonyms Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3
Description TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules that a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that co-express PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens.
Recombinant Mouse TIM-3 Fc Chimera produced in HEK293 cells is a polypeptide chain containing 406 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rmTIM-3 has a molecular mass of 62 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Source HEK293
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized human Galectin at 0.5 μg/mL (100 μL/well) can bind TIM-3 Fc, Mouse(Cat.No.Z03401) with a linear range of 0.78-6.25 μg/mL. Background was subtracted from data points before curve fitting.
Sequence
Leu²²-Ala¹⁹³ (Accession #: Q8VIM0-1), expressed with a C-terminal human IgG1 Fc fragment.
LENAYVFE VGKNAYLP CSYTLSTP GALVPMCW GKGFCPWS
QCTNELLR TDERNVTY QKSSRYQL KGDLNKGD VSLIIKNV
TLDDHGTY CCRIQFPG LMNDKKLE LKLDIKAA KVTPAQTA
HGDSTTAS PRTLTTER NGSETQTL VTLHNNNG TKISTWAD
EIKDSGET IRTA

Molecular Weight 62 kDa, observed by reducing SDS-PAGE.
Purity > 97% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant TIM-3 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse TIM-3 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.


Cat. No.
Product Name
Price
A01835-40
Human TIM3 Antibody (TI.H3), mAb, Mouse - 40 ug
$99.00
A01834-40
Human TIM3 Antibody (TI.B2), mAb, Mouse - 40 ug
$99.00
 


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Cat. No. Z03401
Size
Price
100 ug $250.00
1 mg $1500.00