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Home » Gene Synthesis & Molecular Biology Services » B7-1(CD80) Fc Chimera, Human

B7-1(CD80) Fc Chimera, Human

Synonyms CD80; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7
Description B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature human B7-1 consists of a 208 amino acid extracellular domain (ECD) with two immunoglobulin-like domains, a 21 amino acid transmembrane domain, and a 25 amino acid cytoplasmic domain. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and sCTLA-4. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Recombinant Human B7-1(CD80) Fc Chimera produced in HEK293 cells is a polypeptide chain containing 443 amino acids with the C-terminal human IgG1 Fc fragment. A fully biologically active molecule; rhB7-1(CD80) a molecular mass of 70 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P33681
Source HEK293
Biological Activity Activity 1:Immobilized CTLA 4 Fc Chimera, Human(Cat.No.Z03373) at 2 μg/mL (100 μL/well) can bind biotinylated B7-1(CD80) Fc Chimera, Human with a linear range of 1.22-9.77 ng/mL.
Activity 2:Immobilized CD28 Fc Chimera, Human(Cat.No.Z03413) at 2 μg/mL (100 μL/well) can bind biotinylated B7-1(CD80) Fc Chimera, Human with a linear range of 12.2-390.6 ng/mL.
Sequence
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMV
LTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEG
TYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFE
IPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVS
QDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTF
NWNTTKQEHFPDN

Measured Molecular Weight 75-80 kDa; observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstituted in ddH₂O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant B7-1(CD80), Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution; Human B7-1(CD80) should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.




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Cat. No. Z03409
Size
Price
50 ug $159.00
100 ug $275.00
1 mg $1200.00